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Molecular Diagnostics
Services > Molecular Diagnostics

Diagnostic techniques are in a time of change. Molecular diagnostic techniques have evolved in step with the discoveries of molecular biology over the past twenty-five years. Molecular techniques are more sensitive than classical techniques, and more specific.  The development of these methods was possible due to understanding of the DNA structure and natural replication, transcription and expression mechanisms.

We at RAS constantly working on these techniques bringing out  greater sensitivity and high throughput. It is possible to create assays operating on various taxonomic levels.

RAS Lifesciences provides fast, accurate, cutting-edge Molecular diagnostic tests for patient management and research purposes.

PCR method at Lab is employed for detection of Bacterial, Viral, Fungal, Parasites, Genetic diseases and Cancer. Real Time PCR is used for qualitative and quantitative analysis of DNA and RNA

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Guidelines for specimen collection
Sample Collection device Holding Temperature Comments
Swabs Use viral transport medium Large swab for throat, lesion, etc. Small wire shaft swab for nasopharynx in young children or urethral samples Refrigerate Transport medium with swabs can be obtained from RAS Lifecsiences
Body fluids, BAL, Stool, Urine Use sterile leak proof containers Refrigerate Do not dilute body fluids or BAL in transport medium
Tissues Place in tubes containing liquid transport media to keep tissue moist Refrigerate Viral transport medium can be obtained from RAS Lifecsiences
Blood (Leukocytes or plasma) Collect in EDTA Vacutainers (lavender top tubes). Room temperature Sample must be processed as soon as possible after collection.

Specimen collection instructions for selected specimens
Nasopharynx swab Insert swab into nasopharynx, just past point of resistance. Leave in place for 1 min or rotate to dislodge respiratory epithelial cells; remove and place in transport medium. For small children, thin, flexible wire shaft can be used.
Nasopharynx aspirate Use suction pump connected to a catheter through a mucus trap; catheter should be French gauge 8 for infants, French gauge 12 for adults. Insert catheter as far into nose as possible. Specimen should be taken from posterior part of nasal mucosa which is lined with respiratory epithelium, and not from anterior part which is lined with squamous epithelium. Collect as much of NP secretions as possible; do not dilute sample with saline unless necessary.
Throat swab Swab posteror pharyngeal wall, not buccal mucosa, tonsils, tongue or palate. Swab firmly and thoroughly. Throat swabs are suboptimal for DFA testing due to predominance of squamous instead of respiratory epithelial cells obtained.
Lesion swab Clean lesion with sterile saline soaked gauze pad. Unroof vesicles or remove crusts. Firmly swab base and margins of the lesion, obtaining fluid and cells. After sample collection, clean lesion thoroughly with betadine. Do not use disinfectant prior to sample collection or virus may be inactivated.
Rectal swab Stool specimen required for enteric pathogens; rectal swab to swab rectal mucosa can be done for proctitis.

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